通过对荧光显微光学切片断层成像系统实际成像过程中激光器、液位、平移台等运行状态的分析, 针对性设计了对这些关键部件工作状态进行监测的方法。在此基础上, 进一步研制了一套可远程监测成像系统工作状态的装置, 实现了对成像系统中激光器、液位以及平移台等实时状态的监测, 将成像系统从开环工作状态转变为了带实时反馈的可监控工作状态。远程监控装置的使用提高了荧光显微光学切片断层成像系统的实用性与稳定性, 提高了所获取数据的完整性。

Identification of motor and sensory nerves is important in applications such as nerve injury repair. Conventional practice relies on time consuming staining methods for this purpose. Here, we use laser scanning infrared differential interference contrast (IR-DIC) microscopy for label-free observation of the two types of nerve. Ventral and dorsal nerve roots of adult beagle dogs were collected and sections of different thicknesses were imaged with an IR-DIC microscope. Different texture patterns of the IR-DIC images of the motor and sensory nerve can be distinguished when the section thickness increases to 40 μm. This suggests that nerve fibers in motor and sensory nerves have different distribution patterns. The result hints a potential new way for more rapid identification of nerve type in peripheral nerve repair surgery.

As a second messenger in signal transduction, calcium ion plays a very important role in neuronal information processing and integrating. Limited by the imaging technique, it is difficult to simultaneously perform deep tissue imaging and measure intracellular free calcium concentration ([Ca²+]_i) in different compartments of neurons in brain slice noncollinearly. By means of random access two-photon microscopy, which provides high optical penetration into tissues and low photo damage, we successfully measured [Ca²+]_i of different parts of pyramidal neurons in neocortical layer V in rat brain slices with high spatial and temporal resolution. Combining the patch clamp technique, we stimulated the soma with depolarizing current and explored the dynamics of calcium in pyramidal neurons.